In their effort to help design new diagnostics and vaccines for tuberculosis (TB), Cecilia Lindestam Arlehamn and her co-workers performed a proteome-wide screening of TB derived peptides in patients with active TB vs patients with latent infection using an IFNγ and IL-17 Fluorospot assay and Mabtech IRIS.
The comparison revealed that patients with active TB recognize different epitopes than otherwise healthy latent infected patients. With this information, the research group developed a peptide pool consisting of epitopes exclusively recognized by participants with active TB, allowing distinguishing patients with active disease from latent infections.
A peptide pool like this will be important for measuring correlates of protection in vaccine development, as well as a great possible tool for novel diagnostic assays.
Dr. Cecilia Lindestam Arlehamn