Succeed with your ELISA experiment
We love to see you succeed in your ELISA experiment. Our step-by-step guide will assist you in getting it right. Additionally, our ELISA troubleshooting guide will help you to avoid and resolve errors. Ultimately, we are always here to help you. If you have any questions or are looking for assistance, please don't hesitate to contact us.
ELISA applications
Our ELISAs help you understand immune responses by analyzing a specific cytokine, chemokine, or immunoglobulin. This will help you understand the biological response to different conditions or treatments.
For example, IFN-γ is a typical marker for a Th1 response, but we offer various markers for the different branches of a T cell response. Analyzing different immunoglobulins will help you understand a B cell response. If you are interested in uncovering inflammatory responses, IL-1β, IL-6, or TNF-α could be of interest. But we also cover other biomarkers, such as apolipoproteins.
Check out our publication database, read a summary of our favorite ELISA papers, or find out which sample types were successfully analyzed with our ELISA kits to get inspired by how other researchers have used our ELISA products.
Do you trust your ELISA data?
Detect native proteins
We put considerable effort into characterizing our antibodies and finding a perfectly matched antibody pair. The use of monoclonal antibodies makes our ELISAs specific, reproducible, and sensitive. We don’t cut corners during assay development and always validate that our ELISAs recognize the native protein, i.e., those actually secreted from cells. This makes our ELISAs ready for the reality researchers are uncovering.
Prevent false-positive results
Our ELISAs are sandwich ELISAs, and therefore, the risk for interference by heterophilic antibodies must be considered. These antibodies might crosslink the assay antibodies and lead to false-positive results. For this reason, we developed a sample dilution buffer, called ELISA diluent, that reduces heterophilic antibody interference. This buffer is included in our ELISA Pro kits but can also be purchased separately.
Samples from patients containing rheumatoid factor are known to have an increased risk of generating false-positive results in ELISA. For such clinical samples, we developed specialized kits to withstand heterophilic interference even more efficiently. We call this kit format ELISA PathRF. Learn more about when to choose our ELISA PathRF kits.
Which ELISA kit to choose?
We understand that every project has its own set of requirements. That's why we offer a range of ELISA kits to fit your unique needs. Our kits are designed to deliver accurate and reliable results, every time.
Our ELISA kit formats
ELISA Flex
Flex kits are our most adaptable kit format, allowing you to customize them to your liking. Add your preferred substrate, plates, and buffers to complete this setup.
ELISA Pro
Use our ELISA Pro kits to minimize variability between different experiments. These kits are thoroughly validated and include every reagent necessary.
ELISA PathRF
We recommend using our ELISA PathRF kits when analyzing clinical samples, especially when working with specimens from patients with autoimmune diseases, as these kits are optimized to resist interference by rheumatoid factor (RF) and heterophilic antibodies.
Comparing our ELISA kits
Reactivities and target analytes
|
| Human ELISA kits | ||
|
apoA1
apoB
apoD
apoE
apoH
apoM
CCL2 (MCIP‑1)
CCL22 (MDC)
CD25 (IL‑2Rα)
EBI3
GM‑CSF
Granzyme A
Granzyme B
IFN‑α pan
IFN‑γ
IgA
IgE
IgG
IgM
IL‑1α
IL‑1β
IL‑2
IL‑3
IL‑4
IL‑5
IL‑6
IL‑8 (CXCL8)
IL‑10
IL‑12 (p70)
IL‑12/IL‑23 (p40)
IL‑13
IL‑17A
IL17A/F
IL‑17F
IL‑21
IL‑22
IL‑23
IL‑27
IL-29 (IFN‑λ1)
IL‑31
Perforin
TGF‑β1 (Latent)
TNF‑α
Thioredoxin‑1
| |||
|
| Non-human primate | ||
|
apoA1
apoB
apoH
apoM
CCL2 (MCIP‑1)
CCL22 (MDC)
GM‑CSF
Granzyme B
IFN‑α pan
IFN‑γ
IgA
IgG
IgM
IL‑2
IL‑4
IL‑5
IL‑6
IL‑8 (CXCL8)
IL‑10
IL‑12 (p70)
IL‑12/IL‑23 (p40)
IL‑13
IL‑17A
IL‑21
IL‑22
IL‑23
Perforin
TGF‑β1 (Latent)
TNF‑α
| |||
|
| Mouse | ||
|
apoA1
apoE
IFN‑α pan
IFN‑γ
IgA
IgE
IgEa
IgG
IgM
IL‑1α
IL‑1β
IL‑2
IL‑4
IL‑5
IL‑6
IL‑10
IL‑12 (p70)
IL‑12/IL‑23 (p40)
IL‑13
IL‑17A
IL‑22
TNF‑α
| |||
|
| Cow | ||
|
IFN‑γ
IgG
IL‑2
IL‑4
IL‑5
IL‑8 (CXCL8)
IL‑17A
TNF‑α
| |||
|
| Pig | ||
|
IFN‑γ
IgG
IL‑2
IL‑5
IL‑17A
TNF‑α
| |||
|
| Sheep | ||
|
IFN‑γ
IL‑4
IL‑5
IL‑17A
| |||
|
| Horse | ||
|
IFN‑γ
IgG
IL‑10
| |||
|
| Dog | ||
|
IFN‑γ
IL‑8 (CXCL8)
IL‑10
| |||
|
| Ferret | ||
|
IFN‑γ
IL‑2
| |||
|
| Chicken | ||
|
IFN‑γ
| |||
|
| Cotton rat | ||
|
IFN‑γ
| |||
|
| Llama | ||
|
IFN‑γ
| |||
|
| Rhinoceros | ||
|
IFN‑γ
| |||
Customer voices and award-winning ELISAs
“Mabtech service is highly customer-friendly and efficient. We have been using Mabtech ELISA kits for the past eight years with full satisfaction. Furthermore, I find Mabtech products the best for even developing my in-house ELISA.”
“Mabtech ELISA kits are simply the best. Fast, reproducible, and cost-effective.”
