B cells

Immunoglobulin secretion can be uncovered using ELISpot and FluoroSpot, and even B memory cells can be investigated by turning them into in antibody-secreting cells through a preceding stimulation step. ELISA can be used as a complementing method to quantify the levels of total or antigen-specific immunglobulins. 

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.