ELISA Pro: Mouse IL-5

ELISA Pro: Mouse IL-5

3391-1HP-1

Publications: 22
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAbTRFK4, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IL-5 ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb TRFK5)
Also providedAdhesive plate covers
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse IL-5 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Mouse IL-5
ApplicationELISA
AnalyteIL-5
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-5.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity1 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of mouse Interleukin-5 (IL-5) ELISA Pro kit.
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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell typeT cell, Th2, NK cell
Gene ID16191