ELISA Pro: Mouse IFN-γ

ELISA Pro: Mouse IFN-γ

3321-1HP-1

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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAbR4-6A2, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IFN-γ ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb AN18)
Also providedAdhesive plate covers
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse IFN-γ in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Mouse IFN-γ
ApplicationELISA
AnalyteIFN-γ
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity1 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 8 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of mouse IFN-γ (Interferon-gamma) ELISA Pro kit.

Tutorial, Published November 28, 2022

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Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978