Anti-human/monkey TNF-α mAb (MT15B15), biotin

Anti-human/monkey TNF-α mAb (MT15B15), biotin

3512M-6-250

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Content

Monoclonal antibody MT15B15, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human/monkey TNF-α mAb (MT21A8), unconjugated
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3512M-3-250

Product specifications

Intended use

This monoclonal antibody enables specific detection of monkey TNF-α in immunoassays such as ELISpot, FluoroSpot, ELISA, flow cytometry, and Western blot.

Serum/Plasma samples

Recommendation

MT15B15 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT21A8 (product code 3512M-3). The antibody is also suitable for Western blot and Flow cytometry.

Product details

ProductAnti-human/monkey TNF-α mAb (MT15B15), biotin
ApplicationELISpot, FluoroSpot, ELISA, Flow cytometry, Western blot
AnalyteTNF-α
AntibodyMT15B15
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human TNF-α
HostMouse
IsotypeIgG1
ReactivityNon-human primates
Specificity

Native TNF-α from rhesus and cynomolgus macaques. Native and recombinant human TNF-α.

Cross-reactivityPlease visit www.mabtech.com for information on reactivity with other monkey species
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT15B15, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of TNF-α by Western blot. Recombinant TNF-α from different non-human primates (NHP) were produced in EXPI cells. Supernatants were collected and separated on SDS-PAGE. NHP TNF-α were detected by 1 ug/ml MT15B15-biotin followed by SA-HRP and TMB substrate.
Datasheet/ProtocolSDS
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