ELISA Pro: Mouse apoE

ELISA Pro: Mouse apoE

3752-1HP-1

Publications: 4
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAbmE29, biotin
Enzyme conjugateStreptavidin-HRP
StandardNative mouse apoE ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb mE1)
Also providedAdhesive plate covers
Buffers/SolutionsApo ELISA buffer concentate
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse apoE in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Apolipoprotein dilution
Apolipoproteins are present at high levels in serum and plasma. For ELISA quantification, such samples must therefore be diluted 5,000-200,000 times depending on the apolipoprotein analyzed.

Recommendation

Product details

ProductELISA Pro: Mouse apoE
ApplicationELISA
AnalyteapoE
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse apoE.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range1.56-100 ng/ml
Sensitivity1 ng/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of mouse apolipoprotein E (apoE) ELISA Pro kit.
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apoE

Analyte description

Apolipoprotein E (apoE) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In human apoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml.

Alternative namesApolipoprotein E, apoE, apolipoproteinE, apo E, AI255918, Apo-E
Cell typeT cell, Treg, Tfh
Gene ID11816