ELISA Pro: Human IL-10

ELISA Pro: Human IL-10


Publications: 74
Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.


Detection mAb12G8, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-10 ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb 9D7)
Also providedAdhesive plate covers
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

In stock

Delivery 4-9 business days

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Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IL-10 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using from healthy human blood donors.


Product details

ProductELISA Pro: Human IL-10
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-10.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity1 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of human Interleukin-10 (IL-10) ELISA Pro kit.

Tutorial, Published November 28, 2022

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Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID3586
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