ELISA PathRF: Human TNF-α

ELISA PathRF: Human TNF‑α


Publications: 92
Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.


PlatePre-coated ELISA strip plate (mAb MT25C5)
Detection mAbMTR-9, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human TNF-α ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsRF-block diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human TNF-α in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.


Product details

ProductELISA PathRF: Human TNF-α

This kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage


Shipped at ambient temperature.


Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity2 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human TNF-α.

Highlighted research, Published July 24, 2023

Case report: How researchers used our TNF-α ELISA to evaluate endotoxin contaminations
Bengt Fadeel’s group developed and evaluated a method to uncover endotoxin contaminations using primary human monocyte-derived macrophages and our TNF-α ELISA.
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Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124