ELISA PathRF: Human IL-17A

3520-1HP-DP-1

Publications: 27

Documents

Questions?

Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

Detection mAb	MTR-5, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Recombinant human IL-17A ELISA standard
Substrate	ELISA substrate: TMB for HRP
Plate	Pre-coated ELISA strip plate (mAb MT44.6)
Also provided	Adhesive plate covers
Buffers/Solutions	RF-block diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human IL-17A in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

Product	ELISA PathRF: Human IL-17A
Application	ELISA
Analyte	IL-17A
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-17A.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	3.16-1000 pg/ml
Sensitivity	1 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human IL-17A.

Documents

Datasheet/Protocol
SDS

Human IL-17A/F mAb guide

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Featured product, Published March 1, 2022

When to choose our ELISA PathRF kits

Have you previously encountered false-positive results in your ELISA? Let us present Mabtech's answer for cytokine quantification in plasma and serum containing rheumatoid factor (RF).

Tutorials

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Publications (27)

Loading publications...

Analyte information

IL-17A

Analyte description	Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.
Alternative names	Interleukin 17A, IL-17A, IL17A, CTLA-8, CTLA8, IL-17, IL17, ILA17
Cell type	Th17
Gene ID	3605