ELISA Pro: Bovine IFN-γ

ELISA Pro: Bovine IFN‑γ

3119-1HP-1

Publications: 76
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

PlatePre-coated ELISA strip plate (mAb MT17.1)
Detection mAbMT307, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant bovine IFN-γ ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of bovine IFN-γ in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Bovine IFN-γ
ApplicationELISA
AnalyteIFN-γ
ReactivityCow, Sheep
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IFN-γ.

Cross-reactivity

The mAbs cross-react with IFN-γ from sheep. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity0.5 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 8 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of bovine IFN-γ ELISA Pro kit.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID281237, 443396