Anti-rabbit IgG mAb (MT536), biotin

Anti-rabbit IgG mAb (MT536), biotin

3152-6-250

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Content

Monoclonal antibody MT536, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-rabbit IgG mAb (MT5131), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of rabbit IgG in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT536 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT5131 (product code 3152-3).

Product details

ProductAnti-rabbit IgG mAb (MT536), biotin
ApplicationELISpot, ELISA
AnalyteIgG
AntibodyMT536
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative rabbit IgG
HostMouse
IsotypeIgG1
ReactivityRabbit
SpecificityRabbit IgG
Cross-reactivityNo cross-reactivity (less than 0.1%) with rabbit IgA, IgM, or with IgG from human, monkey, mouse, guinea pig, cow, horse, pig, dog, and cat
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT536, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG
Cell typeB cell