Anti-porcine IFN-γ mAb (pIFNγ-I), unconjugated

Anti-porcine IFN-γ mAb (pIFNγ-I), unconjugated

3130-3-250

Publications: 57
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Content

Monoclonal antibody pIFNγ-I, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Product specifications

Intended use

This monoclonal antibody enables specific detection of porcine IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

pIFNγ-I is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb P2C11 (product code 3130-6).

Product details

ProductAnti-porcine IFN-γ mAb (pIFNγ-I), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIFN-γ
AntibodypIFNγ-I
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant porcine IFN-γ
HostMouse
IsotypeIgG1
ReactivityPig
SpecificityNative porcine (pig) IFN-γ. Recombinant porcine IFN-γ produced in _I-E.coli_-I and human cells. No reactivity with recombinant porcine IFN-γ produced in yeast.
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody pIFNγ-I, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID396991
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