Anti-human IL-4 mAb (IL4-3), PF488P

Anti-human IL-4 mAb (IL4-3), PF488P

3410-71-100T

Publications: 4
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Content

Monoclonal antibody IL4-3, conjugated to PF488P fluorophore. Ready to use.

In stock

Delivery 4-9 business days

Shipping $0.00

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-4-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.

Product details

ProductAnti-human IL-4 mAb (IL4-3), PF488P
ApplicationFlow cytometry
AnalyteIL-4
AntibodyIL4-3
ConjugatePF488P
ClonalityMonoclonal
ImmunogenRecombinant human IL-4
HostRat
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Native and recombinant human IL-4

Cross-reactivityCross-reacts with IL-4 from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Supplied in

PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody IL4-3, conjugated to PF488P fluorophore. Ready to use.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Detection of IL-4 by flow cytometry in viable cynomolgus lymphocytes. Non-stimulated (left panel) and stimulated (PMA/ionomycin; right panel), fixed and permeabilized cells were stained with anti-human IL-4 mAb IL4-3-PF488P in combination with anti-CD3-PE. Flow cytometry was performed on a BD FACSVerse system.

IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1, BCGF1, BSF1
Cell typeT cell, Th2, Treg
Gene ID3565
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