Goat anti-mouse IgG, biotin

3825-6-250

Publications: 20

Documents

Questions?

Validated for our assays.

Content

Goat anti-mouse IgG, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This antibody enables specific detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

Product	Goat anti-mouse IgG, biotin
Application	ELISA, Western blot
Analyte	IgG
Antibody	Goat anti-mouse IgG
Conjugate	Biotin
Clonality	Polyclonal
Immunogen	Native mouse IgG
Host	Goat
Reactivity	Mouse
Specificity	Mouse IgG H-chains
Cross-reactivity	No cross-reactivity with mouse IgA, IgM, human Ig, or non-immunoglobulin serum proteins. Cross-reacts with rat immunoglobulins and may cross-react with immunoglobulins from other species.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Goat anti-mouse IgG, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Product highlights

Featured product, Published June 8, 2023

Publications (20)

Loading publications...

Analyte information

IgG

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Alternative names	Immunoglobulin G, IgG, IgG1, Igh-4, VH7183, 1810060O09Rik, Igh-1, Igh-1a, IgG2b, Igh-3, gamma2b, Igh-1b, IgG3
Cell type	B cell
Gene ID	16017, 380793, 16016, 404711, 380795