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3715-1HP-2

Human apoB ELISA PRO kit

  • Human apoB ELISA kit
  • Standard curve for human apoB ELISAPRO kit.
$495
-+

ELISAPRO kit for quantification of apoB in solution

The apoB ELISAPRO kit provides all necessary reagents and solutions required to quantify apoB in solution, e.g. in cell culture supernatant, and serum/plasma samples. The assay is specific for apoB100 and does not recognize apoB48. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains pre-coated plates and matched amount of reagents and solutions.

Dilution guidelines for serum/plasma samples can be found here.

Pre-coated ELISA plates save time and reduce assay variability.

Species cross-reactivity

The monoclonal antibodies in this kit cross-react with apoB from non-human primates. See the Human/NHP cross-reactivity table.


Analyte Information

ApoB
Apolipoprotein B (apoB, also known as apolipoproteinB, apo B, apoB-100, apoB100 and apo B100) is the main protein component of low density lipoprotein particles (LDL) and it is a large water insoluble molecule of 512 kD (apoB-100) produced mainly in the liver and in the intestine (apoB-48). ApoB is regarded as a negative factor in cardiovascular disease. ApoB-100 is normally 98% of the apoB in human plasma and at a concentration of approximately 0.4-1.3 mg/ml.

Ordering Information

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:
Send your order to order@mabtech.com.

Customers from USA or Canada:
Please send your order to mabtech.usa@mabtech.com.
Application:
  • ELISA
Reactivity:
  • Human / NHP
Documentation: Product datasheet

Product SDS
Contents:
  • Biotinylated detection mAb (LDL 11)
  • Streptavidin-HRP
  • Human apoB ELISA standard
  • Substrate (TMB)
  • Buffers for washing, dilution and standard reconstitution
  • 2 Pre-coated ELISA strip plates (mAb LDL 20/17)
Size: 2 plates
Other formats: Click to view all Human/NHP apoB products
  • Non-Human Primate Cross-reactivity

    The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

    Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Assays with less evidence of cross-reactivity are defined as indications (symbols within parentheses).

     

      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey African Green Monkey Night Monkey* Common Marmoset Squirrel Monkey
    ASSAY Old world New world
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Assay Old world New world
    ApoA1           
    ApoB              
    ApoE (NHP)              
    ApoE            
    ApoH          
    GM-CSF ( ) ( )      
    Granzyme A                
    Granzyme B          
    IFN-α2                
    IFN-α pan       ( )      
    IFN-γ (NHP)
    IFN-γ ( )
    IgA (NHP)              
    IgA              
    IgG              
    IgM              
    Insulin            
    IL-1β                
    IL-2 (NHP)
    IL-4 ( ) ( ) ( ) ( )
    IL-5 ( ) ( ) ( )  
    IL-6 ( ) ( )   ( )  
    IL-8 (NHP)                
    IL-8                
    IL-10              
    IL-12/-23 (p40)            
    IL-12 (p70)                
    IL-13 ( ) ( ) ( )    
    IL-17A (NHP) ( ) ( )      
    IL-17A          
    IL-21            
    IL-22                
    IL-23          
    IL-31                
    Perforin ( )      
    TGF-β1 (latent)              
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
                       
    SINGLE mAbs Old world New world
    SINGLE mAbs                  
    Cell stimulation Old world New world
    Cell stimulation                  
    CD3, mAb CD3-1              
    CD3, mAb CD3-2              
    CD28, mAb CD28-A              
    Flow cytometry Old world New world
    Flow cytometry                  
    Granzyme B, mAb GB11                
    IFN-γ, mAb 1-D1K ( ) ( ) ( ) ( ) ( ) ( )
    IL-2 mAb MT8G10 ( ) ( ) ( ) ( )   ( ) ( ) ( )
    IL-17A mAb MT504                
    Perforin mAb Pf-344          
    Ig reagents Old world New world
    Ig reagents                  
    IgG1 mAb MT1939              
    IgG2 mAb H6200              
    IgG3 mAb MTG34              
    IgG4 mAb MTG42              
    Western blot Old world New world
    Western blot                  
    IFN-γ, mAb MT111W ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( )

    Symbol keys:

    Cross-reactivity

    Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

    • good
    • poor
    • no
    Indications of Cross-reactivity

    Based on reports from others or by analysis using recombinant proteins.

    • ( ) good
    • ( ) poor
    • ( ) no
    Blank boxes

    Represent cross-reactivities not evaluated.

    * Comprises several species. Cross-reactivity may have to be verified on a species basis.

    View human/NHP cross-reactivity table

  • Assay principle

    The ELISA assay explained step-by-step
  • Apolipoprotein Dilution

    Guidelines for dilution of serum/plasma samples for quantification of apolipoproteins in ELISA.

    Apolipoprotein dilution  

  • Analyte Information

    • ApoB
      Apolipoprotein B (apoB, also known as apolipoproteinB, apo B, apoB-100, apoB100 and apo B100) is the main protein component of low density lipoprotein particles (LDL) and it is a large water insoluble molecule of 512 kD (apoB-100) produced mainly in the liver and in the intestine (apoB-48). ApoB is regarded as a negative factor in cardiovascular disease. ApoB-100 is normally 98% of the apoB in human plasma and at a concentration of approximately 0.4-1.3 mg/ml.
  • Ordering Information

    Orders to MabtechOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.


    Email:
    Send your order to order@mabtech.com.

    Customers from USA or Canada:
    Please send your order to mabtech.usa@mabtech.com.
  • Find out more

    Visit our Knowledge Center or see the FAQs.