Anti-human IL-29 mAb (MT6G4), biotin

Anti-human IL-29 mAb (MT6G4), biotin

3570-6-250

Publications: 5
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Validated for our assays.

Content

Monoclonal antibody MT6G4, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-29 in immunoassays such as ELISA, ELISpot, and FluoroSpot.

Serum/Plasma samples

Recommendation

MT6G4 is recommended as detection mAb in combination with capture mAb MT2H7 (product code 3570-3).

Product details

ProductAnti-human IL-29 mAb (MT6G4), biotin
ApplicationELISA, ELISpot, FluoroSpot
AnalyteIL-29 (IFN-λ1)
AntibodyMT6G4
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human IL-29
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Native and recombinant human IL-29

Cross-reactivity

Cross-reacts with human IL-28A and IL-28B.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT6G4, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

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IL-29 (IFN-λ1)

Analyte description

Interleukin 29 (IL-29, IFN-λ1) is at type III interferon. Presently, three type III interferons are described: IFN-λ1, IFN-λ2, and IFN-λ3. Similar to type I interferons, IL-29 is mainly produced by monocytes and dendritic cells in response to viral infections. Among other anti-viral effects, IL-29 induces upregulation of MHC class I on the surface of the virus-infected cell.

Alternative namesInterleukin 29, IL-29, IL29, IFN-λ1, Interferon-λ1, IFN-lamba1, IFNL1
Cell typeT cell, Th1, Th17, NK cell
Gene ID282618
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