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3465-2H

Human Perforin ELISpot BASIC (HRP)

  • Human Perforin ELISpot (HRP)
  • Human Perforin ELISpot (HRP)
  • Non-Human Primate cross-reactivity

Basic ELISpot kit for enumeration of cells secreting Perforin

The human Perforin ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting human Perforin, and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-HRP.

Human Perforin ELISpot kits are also available with Streptavidin-ALP. See Related products.

As complementary products, we offer ELISpot plates and substrates. See Related products.

Species cross-reactivity

The monoclonal antibodies in this kit cross-react with Perforin from non-human primates. See the Human/NHP cross-reactivity table.

Application:
  • ELISpot
Reactivity:
  • Human
  • NHP
Documentation: Product datasheet

Product SDS
Contents:
  • Capture mAbs (Pf-80/164)
  • Biotinylated detection mAb (Pf-344)
  • Streptavidin-HRP
Size: for 4 plates
  • Non-Human Primate Cross-reactivity

    The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

    Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Assays with less evidence of cross-reactivity are defined as indications (symbols within parentheses).

      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey African Green Monkey Night Monkey* Common Marmoset Squirrel Monkey
    Assay Old world New world
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Assay Old world New world
    ApoA1           
    ApoB              
    ApoE (NHP)              
    ApoE            
    ApoH          
    GM-CSF ( ) ( )      
    Granzyme A                
    Granzyme B          
    IFN-α2                
    IFN-α pan       ( )      
    IFN-γ (NHP)
    IFN-γ ( )
    IgA (NHP)              
    IgA              
    IgG              
    IgM              
    IL-1β              
    IL-2 (NHP) ( ) ( ) ( ) ( )   ( ) ( ) ( )
    IL-4 ( ) ( ) ( )  
    IL-5 ( ) ( ) ( )  
    IL-6 ( ) ( )   ( )  
    IL-8 (NHP)                
    IL-8                
    IL-10              
    IL-12/-23 (p40)            
    IL-12 (p70)              
    IL-13 ( ) ( )      
    IL-17A (NHP) ( ) ( )      
    IL-17A          
    IL-21                
    IL-22                
    IL-23          
    IL-31                
    Perforin ( )      
    TGF-β1 (latent)              
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Single mAbs Old world New world
    Single mAbs                  
    CD3, mAb CD3-1              
    CD3, mAb CD3-2              
    CD28, mAb CD28-A              

    Symbol keys:

    Cross-reactivity

    Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

    • good
    • poor
    • no
    Indications of Cross-reactivity

    Based on reports from others or by analysis using recombinant proteins.

    • ( ) good
    • ( ) poor
    • ( ) no
    Blank boxes

    Represent cross-reactivities not evaluated.

    * Comprises several species. Cross-reactivity may have to be verified on a species basis.

    View human/NHP cross-reactivity table

  • Find out more

    Visit our Knowledge Center or see the FAQs.