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3450-2AHuman IL-12/-23 (p40) ELISpotBASIC (ALP)

  • ELISpot kit - a basic kit containing monoclonal capture and detection antibodies and Streptavidin-enzyme conjugate. Color of the vial tops may vary. (Mabtech)
  • PBMC (10,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting IL-12/-23 (p40) was analyzed by ELISpot.
  • Non-Human Primate cross-reactivity
$515
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Basic ELISpot kit for enumeration of cells secreting IL-12/-23 (p40)

The human IL-12/-23 (p40) ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting human IL-12/-23 (p40), and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.

The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISpot assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.

Human IL-12/-23 (p40) ELISpot kits are also available with Streptavidin-HRP. See Related products.

As complementary products, we offer ELISpot plates and substrates. See Related products.

Species cross-reactivity

The monoclonal antibodies in this kit cross-react with IL-12/-23 (p40) from non-human primates. See the Human/NHP cross-reactivity table.

Application:
  • ELISpot
Reactivity:
  • Human
  • NHP
Documentation: Product datasheet
Contents:
  • Capture mAbs (MT86/221)
  • Biotinylated detection mAb (MT618)
  • Streptavidin-ALP
Size: for 4 plates
  • Non-Human Primate Cross-reactivity

    The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

    Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Assays with less evidence of cross-reactivity are defined as indications (symbols within parentheses).

      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Assay Old world New world
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Assay Old world New world
    ApoA1           
    ApoB              
    ApoE (NHP)              
    ApoE            
    ApoH          
    GM-CSF ( ) ( )      
    Granzyme B          
    IFN-α2                
    IFN-α pan       ( )      
    IFN-γ (NHP) ( )  
    IFN-γ ( )
    IgA (NHP)              
    IgA              
    IgG              
    IgM              
    IL-1β              
    IL-2 (NHP) ( ) ( ) ( ) ( )   ( ) ( ) ( )
    IL-4 ( ) ( ) ( )  
    IL-5 ( ) ( ) ( )  
    IL-6 ( ) ( )   ( )  
    IL-8 (NHP)                
    IL-8                
    IL-10              
    IL-12/-23 (p40)            
    IL-12 (p70)              
    IL-13 ( ) ( )      
    IL-17A (NHP) ( ) ( )      
    IL-17A          
    IL-21                
    IL-22                
    IL-23          
    IL-31                
    Perforin ( )      
    TGF-β1 (latent)              
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Single mAbs Old world New world
    Single mAbs                  
    CD3, mAb CD3-1              
    CD3, mAb CD3-2              
    CD28, mAb CD28-A              

    Symbol keys:

    Cross-reactivity

    Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

    • good
    • poor
    • no
    Indications of Cross-reactivity

    Based on reports from others or by analysis using recombinant proteins.

    • ( ) good
    • ( ) poor
    • ( ) no
    Blank boxes

    Represent cross-reactivities not evaluated.

    * Comprises several species. Cross-reactivity may have to be verified on a species basis.

    View human/NHP cross-reactivity table

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