Basic ELISpot kit for enumeration of cells secreting IL-12/-23 (p40)
The human IL-12/-23 (p40) ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting human IL-12/-23 (p40), and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.
The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISpot assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.
Human IL-12/-23 (p40) ELISpot kits are also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
The monoclonal antibodies in this kit cross-react with IL-12/-23 (p40) from non-human primates. See the Human/NHP cross-reactivity table.
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- IL-12/-23 (p40)
Interleukin-12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is responsible for Th17 expansion and stabilization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
- IL-12/-23 (p40)