Anti-human IL-10 mAb (12G8), biotin

Anti-human IL-10 mAb (12G8), biotin

3430-6-250

Publications: 145
Documents
Questions?
Validated for our assays.

Content

Monoclonal antibody 12G8, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 1

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-10 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

12G8 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb 9D7 (product code 3430-3).

Product details

ProductAnti-human IL-10 mAb (12G8), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-10
Antibody12G8
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human IL-10
HostRat
IsotypeIgG2a
ReactivityHuman
SpecificityNative and recombinant human IL-10
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration1 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody 12G8, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Loading publications...

IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID3586
1 / 2