Skip to:

3420-4AST-2Human IFN-γ ELISpotPLUS (ALP), strips

  • ELISpotPLUS kit containing strip-plates pre-coated with monoclonal antibody, detection antibody, Streptavidin-enzyme conjugate and ELISpot substrate. Anti-CD3 mAb is supplied as a positive control for cytokine secretion. An empty plate frame is included. Color of the vial tops may vary.
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of the CEF peptide pool (available from Mabtech, code 3615-1) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of Phytohaemagglutinin (PHA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of Tuberculin purified protein derivative (PPD) and the number of cells secreting IFN-γ was analyzed by ELISpot.
$465
-+

ELISpotPLUS for enumeration of cells secreting IFN-γ

This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ. The kit includes ELISpot 8-well strip plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

Pre-coated plates reduce assay time and minimize variability. In addition, strip-plates are ideal when running a limited number of wells.

Human IFN-γ ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.

Application:
  • ELISpot
Reactivity:
  • Human
Documentation: Product datasheet
Contents:
  • Biotinylated detection mAb (7-B6-1)
  • Streptavidin-ALP
  • Positive control (anti-CD3 mAb)
  • Substrate (BCIP/NBT-plus)
  • 2 Pre-coated clear strip plates (mAb 1-D1K)
Size: 2 plates