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3420-2AHuman IFN-γ ELISpotBASIC (ALP)

  • ELISpot kit - a basic kit containing monoclonal capture and detection antibodies and Streptavidin-enzyme conjugate. Color of the vial tops may vary. (Mabtech)
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of the CEF peptide pool (available from Mabtech, code 3615-1) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of Phytohaemagglutinin (PHA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of Tuberculin purified protein derivative (PPD) and the number of cells secreting IFN-γ was analyzed by ELISpot.
$515
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Basic ELISpot kit for enumeration of cells secreting IFN-γ

The human IFN-γ ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting human IFN-γ, and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.

Human IFN-γ ELISpot kits are also available with Streptavidin-HRP. See Related products.

As complementary products, we offer ELISpot plates and substrates. See Related products.

Application:
  • ELISpot
Reactivity:
  • Human
Documentation: Product datasheet
Contents:
  • Capture mAb (1-D1K)
  • Biotinylated detection mAb (7-B6-1)
  • Streptavidin-ALP
Size: for 4 plates