ELISpot Plus: Human IL-1β (ALP)

ELISpot Plus: Human IL-1β (ALP)

3416-4APW-2

Publications: 3
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The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAb7P10, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, white MSIP (mAb MT175)

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-1β using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

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Product details

ProductELISpot Plus: Human IL-1β (ALP)
ApplicationELISpot
AnalyteIL-1β
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).

Cross-reactivity

The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC were incubated for 20 h in the presence (4000 cells/well) or absence (24,000 cells/well) of R848 (1μg/ml), and the number of cells secreting IL-1β was analyzed by ELISpot.
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell typeMonocyte/MΦ, mDC
Gene ID3553
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