ELISA Flex: Human IL-1β (ALP)

ELISA Flex: Human IL-1β (ALP)

3416-1A-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT175
Detection mAb7P10, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human IL-1β ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-1β in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human IL-1β (ALP)
ApplicationELISA
AnalyteIL-1β
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).

Cross-reactivity

The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range2.5-250 pg/ml
Representative standard curve for human IL-1β ELISA Flex kit using pNPP substrate.
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell typeMonocyte/MΦ, mDC
Gene ID3553
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