ELISA Flex: Bovine IL-4 (HRP)

ELISA Flex: Bovine IL-4 (HRP)

3118-1H-6

Publications: 10
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbbIL4-I
Detection mAbbIL4-II, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant bovine IL-4 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of bovine IL-4 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Bovine IL-4 (HRP)
ApplicationELISA
AnalyteIL-4
ReactivityCow, Sheep
SpecificityThis kit is based on a matched pair of mAbs specific for bovine (cow) IL-4.
Cross-reactivityThe mAbs cross-react with IL-4 from sheep. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range20-2000 pg/ml
Representative standard curve for bovine IL-4 ELISA Flex kit using TMB substrate.
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IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1
Cell typeT cell, Th2, Treg
Gene ID280824, 443327