The enzyme-linked immunospot (ELISpot) assay is an extremely sensitive immunoassay performed in a 96-well plate format to quantify protein-secreting cells. 

The assay principle is straight forward: cells are cultured and stimulated inside a plate coated with capture antibody. Cytokines, immunoglobulins, or other target proteins secreted by the cells are captured immediately upon secretion and throughout the stimulation process. After cell removal, secreted proteins are identified using a detection antibody. Visible spots form after adding a precipitating substrate. Each spot corresponds to an individual analyte-secreting cell.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.