Analyte combinations in FluoroSpot

Originally published November 11, 2022, updated November 30, 2022


Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

AnalytesGM-CSFGzBIFN-γIgAIgGIgMIL-1αIL-1βIL-2IL-3IL-4IL-5IL-6IL-8IL-10IL-12 (p70)IL-12/-23IL-13IL-17AIL-21IL-22IL-27TNF-α
GM-CSF       *            
GzB  *     *    *       *
IFN-γ *          
IgA                     
IgG                     
IgM                     
IL-1α                   
IL-1β*                  
IL-2 *           *  
IL-3                    
IL-4                     
IL-5          *   *  
IL-6                  
IL-8                       
IL-10 *      *     *   
IL-12 (p70)                       
IL-12/-23                      
IL-13       *         *  
IL-17A           *    *  
IL-21                      
IL-22         *     **    
IL-27                      
TNF-α*               

 

Symbol keys:

Verified by Mabtech, mainly with human samples

  •    works well
  • * recommended to include anti-CD28
  •    major capture effects observed
  • Blank boxes indicate combinations not evaluated

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.


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