Basic ELISpot kit for enumeration of cells secreting IFN-γ
The rat IFN-γ ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting rat IFN-γ, and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.
Rat IFN-γ ELISpot kits are also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
- Camara M., et al. CD8 T cell help is required for efficient induction of EAE in Lewis rats Journal of neuroimmunology, 2013
- Foo D. G., et al. T cell monitoring of chemotherapy in experimental rat tuberculosis Antimicrobial agents and chemotherapy 55 3677, 2011
Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.