B-cell ELISpot

In spite of the first described use of the ELISpot being that of detecting antibody-secreting B cells, there have so far not been any designated ELISpot kits for this application. To accommodate this need for validated reagents and protocols we have recently developed a series of kits for detection of human, monkey and mouse Ig-secreting cells.

As schematically illustrated below, the B-cell ELISpot may be used both for determining the number of antigen-specific B cells (A) as well as the total number of antibody-secreting cells (B). In the antigen-specific setting, the assay can be performed in either of two ways, one where the antigen is immobilized on the ELISpot plate and one where the same antigen is instead used for detection. While the first approach has been the one almost exclusively used, we have found that using antigen for detection may have several advantages including better sensitivity and a lesser need for antigen.

A Antigen-specific IgG ELISpot	B Total IgG ELISpot

Click here to find an overview of current available B-cell ELISpot kits.

All B-cell ELISpot kits include ELISpot plates, capture and detection reagents, a polyclonal activator and substrate and can be used both for the analysis of antigen-specific responses and the detection of the total number of Ig secreting cells.