ELISpot kit formats
The ELISpot assay is a valuable method in numerous different settings. Mabtech aims to supply high quality ELISpot reagents for the small frontline research group as well as for the big vaccine trials; accordingly the ELISpot reagents from Mabtech are available in different formats. Our kits have been developed to offer maximum convenience for the researcher. In addition, the same monoclonal antibodies can be obtained as separate reagents as well as matched antibody pairs with equal performance. The most advanced ELISpot kit from Mabtech is our ELISpotPRO kit. Beginners in the ELISpot field will find top performance and easy handling with these kits, as will the users looking for short assay time and validated high reproducibility. Other users who would like to optimize their own assays often choose our basic ELISpot kit. Mabtech also supplies the ELISpotPLUS kit, a complete ELISpot kit including plates and substrate. In the table below you will find a list of the specific contents of each kit. Now, as the first company, we are able to offer the ELISpotPLUS kit as pre-coated plates in strip-plate format (12 strips with 8 wells on each strip). This allows the user to run one plate at multiple time points and is ideal when e.g. having only one or a few samples to test. Please contact Mabtech for further details and check our overview page for information about which ELISpot kit formats are available for specific cytokines or other analytes. On request pre-coated plates for other cytokines than those listed may be obtained.
Contents in Mabtech ELISpot kits
|
Contents |
ELISpotPRO
|
ELISpotPLUS
|
ELISpot kit
|
|
precoated
|
regular
|
strips
|
| PVDF strip-plates precoated with capture mAb |
|
|
|
|
|
| PVDF plates precoated with capture mAb |
|
|
|
|
|
| PVDF plates |
|
|
|
|
|
| Capture mAb* |
|
|
|
|
|
| One-step detection mAb-enzyme |
|
|
|
|
|
| Biotinylated detection mAb |
|
|
|
|
|
| Streptavidin-enzyme conjugate |
|
|
|
|
|
| Substrate |
|
|
|
|
|
| Positive control or activating reagent |
|
|
|
|
|
*The kit contains matched quantities of coating and detection antibodies sufficient for 4 plates when using the recommended coating concentration of capture antibody. |