Additional material required: Plates, buffers and substrate Estimated assay time: 20 hours including overnight coating but excluding cell-incubation time.

ELISpot kit

This basic kit includes the essential reagents to set up an ELISpot assay. It leaves the choice of plates and substrate to the user.

This kit can be supplied in larger sizes on request.

Kit contents (reagents sufficient for at least 4 plates):

• Capture mAb
• Biotinylated detection mAb
• Streptavidin-enzyme conjugate (ALP or HRP)