ELISA for direct measurement of human latent TGF-▀1
Latent TGF-▀1 ELISA
Mabtech has launched a new ELISA assay for direct quantification of latent TGF-▀1 in plasma or cell culture supernatants.
Increased Ease of Use
Transforming Growth Factor β1 (TGF-β1) is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). This is also true for the other latent TGF-β isoforms, and these latency complexes of TGF-β have been shown to be the most commonly found forms of the molecules.
Conventional measurements of human latent TGF-β1 by conventional TGF-β1 ELISA rely on a sample pre-treatment step to dissociate the latency complex, i.e. sample acidification and subsequent neutralization, prior to analysis.
Mabtechĺs novel Latent TGF-β1 ELISA is based on monoclonal antibodies directed to the LAP entity of latent TGF-β1. This enables a direct analysis of latent TGF-β1 with no need for sample pre-treatment.
Highly Efficient and Accurate Measurements
Results of latent TGF-β1 levels measured in human plasma directly by our new Latent TGF-β1 ELISA have been shown to correlate well with conventional analyses of acid-treated samples by TGF-β1 ELISA.
Specificity of Measurements
The Latent TGF-β1 ELISA has been demonstrated not to cross-react with the human latent TGF-β2 and -β3 isoforms. Also, in contrast to human TGF-β1 ELISAs, which inevitably cross-react with bovine TGF-β1 due to high evolutionary conservation, the Latent TGF-β1 ELISA does not cross-react with bovine TGF-β1. The Latent TGF-β1 ELISA is therefore an ideal solution for analysis of human latent TGF-β1 in cell culture supernatants containing bovine serum supplements.
Latent TGF-β1 ELISA can be also used to quantify latent TGF-β1 levels in rhesus and cynomolgus macaques, due to the conservation across primate species.
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Reference: Arestrom et al. Measurement of Human Latent Transforming Growth Factor-β1 using a Latency Associated Protein-Reactive ELISA J. Immunol. Methods, 379: 23-29, 2012