The Enzyme-Linked ImmunoSpot (ELISpot) assay is a very sensitive immunoassay, allowing the detection of a secreted cytokine at the single cell level. With detection levels that can be as low as one cell in 100 000, the ELISpot is one of the most sensitive cellular assays available. Depending on the substance analyzed, it is between 20 and 200 times more sensitive than a conventional ELISA.
